Discerning the regulated wound healing potential of Ocimum americanum by probing the rosmarinic acid content-a paradigm on zebrafish caudal fin regeneration

Trends in Phytochemical Research

دوره 6، شماره 2، شهریور 2022، صفحه 106-115   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.30495/tpr.2022.691368

نویسندگان

Arockiya Anita Margret^* ؛ Ramasamy Maheswari؛ Arokiaraj Sherlin Rosita؛ Edward Jesucastin

Department of Biotechnology and Bioinformatics, Bishop Heber College, Tiruchirappalli -620017, Tamil Nadu, India

چکیده

Acute traumatic wounds create a serious impact on pathology and impede recovery management. This work emphasis on the wound healing potential of Ocimum americanum extract by caudal regeneration studies in zebrafish model. Molecular docking studies exhibited high binding energy (-8.8 kcal/mol) and an inhibitory effect was conferred between the plant’s standard compound rosmarinic acid against gelatinase. To evaluate its efficacy the plant extract is queried for the presence of phenolic components analogous to rosmarinic acid. The FTIR studies showed similar functional groups with the incidence of phenolic contents and the HPLC assay validated its presence by depicting a communal peak at 4.19 min. In vivo studies authenticated the LC₅₀ value at an optimum concentration of 135.51 ± 0.01 mg/L. The caudal fins were excised to assay regeneration capacity and histopathology sections augmented restoration. Together these results conclude the wound healing capability O. americanam with the probability to regenerate caudal fins and reparation.
 

کلیدواژه‌ها

Caudal regeneration؛ Matrix metalloproteinases؛ Ocimum americanum؛ Rosmarinic acid؛ Wound healing؛ Zebrafish

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