Micropropagation of English Yew, an Ornamental-Medicinal Tree

Journal of Ornamental Plants

مقاله 1، دوره 12، شماره 2، شهریور 2022، صفحه 91-99   

نوع مقاله: Research Paper

نویسندگان

Ali Sahari Moghadam¹؛ Behzad Kaviani^{* 2}؛ Ali Mohammadi Torkashvand¹؛ Vahid Abdossi¹؛ Alireza Eslami²

¹Department of Horticultural Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran

²Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran

چکیده

An in vitro propagation method of English yew (Taxus baccata L.) through organogenesis method using kinetin (Kin) and indole 3-butyric acid (IBA) as plant growth regulators and apical bud as explant is presented. Apical buds excised from mother plants were inoculated on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of Kin (0.00, 0.50, 1.00 and 2.00 mg l^–1) as a cytokinin and IBA (0.00, 0.50, 1.00 and 2.00 mg l^–1) as an auxin. Results showed that the highest number of node (6.75) was obtained on MS medium containing 2.00 mg l^–1 Kin. The maximum shoot number (5.00) was obtained on MS medium supplemented with 1.00 mg l^–1 Kin together with 1.00 mg l^–1 IBA. The largest number of root (6.50) was produced on explants grown on medium enriched with 2.00 mg l^–1 Kin together with 1.00 mg l^–1 IBA. Plantlets were transferred to pots filled with perlite and peat moss in equal proportions for acclimatization. These plantlets were acclimated and successfully established in cultivation beds. .

کلیدواژه‌ها

Forest trees؛ In vitro multiplication؛ Plant growth regulators؛ Threatened ornamental plant

مراجع

Abbasin, Z., Zamani, S., Movahedi, S., Khaksar, G. and Sayed Tabatabaei, B.E. 2010. In vitro micropropagation of yew (Taxus baccata) and production of plantlets. Biotechnology, 9 (1): 48–54.

Adibi Baladeh, D. and Kaviani, B. 2021. Micropropagation of medlar (Mespilus germanica L.), a Mediterranean fruit tree. International Journal of Fruit Science, 21 (1): 242–254.

Alvarez, J.M., Majada, J. and Ordas, R.J. 2009. An improved micropropagation protocol for maritime pine (Pinus pinaster) isolated cotyledons. Forestry, 10: 175–184.

Anderson, E.D. and Owens, J.N. 2001. Embryo development, megagametophyte storage product accumulation and seed efficiency in Taxus brevifolia. Canadian Journal of Forestry Research, 31: 1046–1056.

Brickell, C. and Zuk, J.D. 1997. A-Z Encyclopedia of Garden Plants. DK Publishing, Inc. New York, USA. 1095 p.

Campbell, M.M., Brunner, A.M., Jones, H.M. and Strauss, S.H. 2003. Forestry’s fertile crescent: The application of biotechnology to forest trees. Plant Biotechnology Journal, 1 (3): 141–154.

Castillón, J. and Cornish, K.A. 2000. Simplified protocol for micropropagation of guayule (Parthenium argentatum Gray). In vitro Cellullar and Developmental Biology Plant, 36: 215–219.

Chand, S. and Singh, A.K. 2004a. In vitro shoot regeneration from cotyledonary node explants of a multipurpose leguminous tree, Pterocarpus marsupium Roxb. In vitro Cellular and Developmental Biology- Plant, 40: 167–170.

Chang, S.H., Ho, C.K., Chen, Z.Z. and Tsay, Y.J. 2001. Micropropagation of Taxus mairei from mature trees. Plant Cell Report, 20: 496–502.

Chang, S.H., Ho, C.K. and Tsay, J.Y. 1998. Micropropagation of Taxus mairei seedlings at different ages and recoverability of their plagiotropic shoots. Taiwan Journal of Forest Science, 13: 29–39.

Chang, S.H., Ho, C.K., Chen, Z.Z. and Tsay, Y.J. 2001. Micropropagation of Taxus mairei from mature trees. Plant Cell Report, 20: 496–502.

Dhar, U., Upreti, J. and Bhatt, I.D. 2000. Micropropagation of Pittosporum napaulensis (DC.) Rehder & Wilson – A rare, endemic Himalayan medicinal tree. Plant Cell, Tissue and Organ Culture, 63: 231–235.

Dinesh, R.M., Patel, A.K., Vibha, J.B., Shekhawat, S. and Shekhawat, S.N. 2019. Cloning of mature pomegranate (Punica granatum) cv. Jalore seedless via in vitro shoot production and ex vitro rooting. Vegetos, 32: 181-189.

Ewald, D. 2007. Micropropagation of yew (Taxus baccata L.). In: S.M. Jain and H. Häggman (eds.), Protocols for Micropropagation of Woody Trees and Fruits, 117–123, Springer.

Fan, S., Jian, D., Wei, X., Chen, J., Beeson, R.C., Zhou, Z. and Wang, X. 2017. Micropropagation of blueberry ‘Bluejay’ and ‘Pink Lemonade’ through in vitro shoot culture. Scientia Horticulturae, 226 (19): 277–284.

Kalinina, A., Daniel, C. and Brown, W. 2007. Micropropagation of ornamental Prunus spp. and GF305 peach, a Prunus viral indicator. Plant Cell Report, 26: 927–935.

Kaviani, B. and Negahdar, N. 2017. Propagation, micropropagation and cryopreservation of Buxus hyrcana Pojark., an endangered ornamental shrub. South African Journal of Botany, 111: 326–335.

Kereša, S., Bošnjak, A.M., Barić, M., Jerčić, I.H., Šarčević, H. and Biško, A. 2012. Efficient axillary shoot proliferation and in vitro rooting of apple cv. ‘Topaz’. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 40 (1): 113–118.

Mahipal, S., Shekhawat, N.S. and Manokari, M. 2016. In vitro propagation, micromorphological studies and ex vitro rooting of cannon ball tree (Couroupita guianensis Aubl.): A multipurpose threatened species. Physiological and Molecular Biology Plants, 22 (1): 131–142.

Majada, J.P., Sierra, M.I. and Sanchez-Tames, R. 2000. One step more towards taxane production through enhanced Taxus propagation. Plant Cell Report, 19: 825–830.

Metaxas, D., Syros, T., Yupsanis, T. and Economou, A. 2004. Peroxidases during adventitious rooting in cuttings of Arbutus unedo and Taxus baccata as affected by plant genotype and growth regulator treatment. Plant Growth Regulation, 44: 257–266.

Mitchell, A.K. 1997. Propagation and growth of pacific yew (Taxus brevifolia Nutt.) cuttings. Northwest Science, 71: 56–63.

Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiology Plant, 15: 473–479.

Noroozi Sharaf, A.R., Hamidoghli, Y. and Zakizadeh, H. 2011. In vitro seed germination and micropropagation of primrose (Primula heterochroma Stapf.) an endemic endangered Iranian species via shoot tip explants. Horticulture, Environment, and Biotechnology, 52 (3): 298–302.

Nunes, S., Sousa, D., Pereira, V.T., Correia, S., Marum, L., Santos, C. and Dias, M.C. 2018. Efficient protocol for in vitro mass micropropagation of slash pine. In vitro Cellular and Developmental Biology Plant, 54 (2): 175–183. 

Onay, A. 2000. Micropropagation of Pistachio from mature trees. Plant Cell, Tissue and Organ Culture, 60: 159–162.

Prakash, E., Sha Valli Khan, P.S., Vivek Sreenivasa Rao, T.J. and Meru, E.S. 2006. Micropropagation of red sanders (Pterocarpus santalinus L.) using mature nodal explants. Journal of Forestry Research, 11, 329–335.

Romano, A., Barros, S. and Martinus-Lucao, M.A. 2002. Micropropagation of the Mediterranean tree Ceratomia siliqua. Plant Cell, Tissue and Organ Culture, 68: 35–41.

Rathore, J.S., Rathore, V., Shekhawat, N.S., Singh, R.P., Liler, G., Phulwaria, M. and Dagla, H.R. 2004. Micropropagation of Woody Plants. pp. 195-217. In: Srivastava, P.S., Narula, A., Srivastava, S. (eds.). Plant Biotechnology and Molecular Markers. Anamaya Publishers, New Delhi, India.

Savita, V., Virk, G. and Nagpal, A. 2010. Effect of explant type and different plant growth regulators on callus induction and plantlet regeneration in Citrus jambhiri Lush. Environment & We an International Journal of Science & Technology, 5: 97–106.

Sharma, H. 2017. Role of growth regulators in micropropagation of woody plants - A review. International Journal of Advances Research, 5: 2378–2385.

Sharma, U., Kataria, V. and Shekhawar, N.S. 2017. In vitro propagation, ex vitro rooting and leaf micromorphology of Bauhinia racemosa Lam.: A leguminous tree with medicinal values. Physiological, Molecular and Biological Plants, 23 (4): 969–977.

Sharma, H. and Vashistha, B.D. 2015c. In vitro plant regeneration through callus in giloy (Tinospora cordifolia (Willd.) Miers ex Hook. F & Thoms.). Indian Journal of Science, 12 (34): 59–68.

Sulusoglu, M. and Cavusoglu, A. 2013. Micropropagation of cherry laurel Prunus laurocerasus L. Journal of Food, Agriculture and Environment, 11: 576–579.

Takihira, M., Otani, M., Tsuchiya, S. and Shimada, T. 2007. Plant regeneration from leaf explants of auricula cultivars (Primula × pubescens Jacq.). Plant Biotechnology, 24: 425–427.

Venkatachalam, P., Kalaiarasi, K. and Sreeramanan, S. 2015. Influence of plant growth regulators (PGRs) and various additives on in vitro plant propagation of Bambusa arundinacea (Retz.) Wild: A recalcitrant bamboo species. Journal of Genetic Engineering and Biotechnology, 13: 193–200. 

Yildirim, H. 2012. Micropropagation of Pistacia lentiscus L. from axenic seedling-derived explants. Scientia Horticulturae, 137 (1): 29–35.