The effects of low-power laser on the promotion of spermatogenesis in a mouse model of azoospermia (in-vivo)

International Journal of Biophotonics and Biomedical Engineering

دوره 2، شماره 1، دی 2022، صفحه 15-30   

نوع مقاله: Research article

شناسه دیجیتال (DOI): 10.30495/ijbbe.2022.698214

نویسندگان

Habib Tajalli^{* 1}؛ Masoud Maleki²؛ Esmail Safavi³؛ Reza Shahi⁴؛ Fatemeh Firoozi²؛ Zahra Akbarpour²؛ Alireza Sotoudeh Khyaban⁵

¹Biophotonics Research Center,Tabriz Branch, Islamic Azad University

²Department of Basic Science, Biotechnology Research Center, Tabriz Branch, Azad Islamic University, Tabriz, Iran

³Department of Basic Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Azad Islamic University, Tabriz, Iran

⁴Department of Mathematics and Statistics, Tabriz branch, Islamic Azad University, Tabriz, Iran

⁵Research Center of Biophtonics, Islamic Azad university, Tabriz Branch, Tabriz, Iran

چکیده

In this paper we investigate the effect of low power laser on spermatogenesis in testicular tissue of azoospermia mouse model in-vivo. In this experimental work, 112 adult male Syrian mice were randomly divided into three main groups: negative control group, positive (Azoospermia control) group, and experimental group, but to determine the best dose of laser radiation three experimental groups were tested. To create azoospermia control group, Busulfan was used at a dose of 30mg/kg, for 21 days by intraperitoneal injection. In the experimental groups after Busulfan treatment, they were applied by the low power diode laser (wavelength of 808nm) with three different energy densities of 2, 4, and 8 J/cm2. The employment of a laser with an energy density of 8 J/cm2 was shown to be beneficial in boosting germ cell and sperm production.

کلیدواژه‌ها

spermatogenesis؛ azoospermia؛ laser therapy؛ infertility

مراجع

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