Bioinformatic analysis of differential expression of pyroptosis related genes in cerebral infarction

International Journal of Industrial Chemistry

دوره 13، شماره 1، خرداد 2022

نوع مقاله: research article

شناسه دیجیتال (DOI): 10.47176/IJIC.13.1.7

نویسندگان

Renzhi Deng¹؛ Jianying Chen²؛ Xianhui Pang¹؛ Xu Han¹؛ Chunhai Tang^{* 1}

¹Department of Neurosurgery, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530000, China

²Department of Operation Room, The Second Nanning People's Hospital, Nanning, Guangxi 530000, China

چکیده

Cerebral infarction is also called ischemic stroke, is caused by various causes of local brain tissue regional blood supply disorders, resulting in brain tissue ischemic hypoxic lesion necrosis. Pyroptosis has been demonstrated in recent years to be an inflammatory form of programmed cell death. However, the expression of pyroptosis-related genes in cerebral infarction is obscure. The GSE122709 dataset was separated into cerebral infarction group and healthy control group. Eleven differentially expressed pyroptosis-related genes were selected, including CASP3, CASP9, ELANE, GPX4, GSDMD, NLRC4, PYCARD and SCAF11 were significantly down-regulated while GSDMB, NLRP1 and TIRAP were significantly up-regulated in cerebral infarction group in comparison with the controls. Go enrichment and KEGG pathway analysis have been performed for the differentially expressed pyroptosis-related genes. The outcomes showed that, in terms of biological process, most of pyroptosis-related DEGs were involved in the regulation of metabolic process, cellular compaonent organization, multicellular organismal process, response to stimulus, multi-organism process and biological regulation. In terms of cellular component, most of pyroptosis-related DEGs were implicated in cytosol and protein-containing complex, nucleus and membrane-enclosed lumen. In terms of molecular function, most of pyroptosis-related DEGs were involved in protein binding, and ion binding. Furthermore, we analyzed the enriched pathway of 11 pyroptosis-related DEGs, and displayed the mostly enriched pathway of pyroptosis-related DEGs (CASP9, CASP3, NLRC4 and PYCARD), which included mitochondrial apoptotic pathway. In conclusion, pyroptosis related genes may function as biomarkers for the cerebral infarction patients and may also serve as potential therapeutic targets.

کلیدواژه‌ها

Cerebral infarction؛ pyroptosis؛ GO؛ KEGG, bioinformatics