Mechanism of antinociceptive activity of the methanol leaf extract of Senna italica (Mill) in murine model of pain

Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

دوره 14، شماره 1، مرداد 2023، صفحه 85-95   

نوع مقاله: Original article

شناسه دیجیتال (DOI): 10.30495/medherb.2023.704606

نویسندگان

Amina Busola Olorukooba^{* 1}؛ Asmau Nasir Hamza²؛ Aisha Oiza Musa³

¹Department of Pharmacology and Therapeutics, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria, Kaduna State-Nigeria;

²Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria, Kaduna State-Nigeria;

³Department of Botany, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Kaduna State-Nigeria;

چکیده

Background & Aim: Senna italica leaf (SIL) is extensively used in traditional medicine for the management of various types of pain including stomach cramps, back ache, joint pains, headache, and migraine. The current study was designed to scientifically investigate the purported uses of the leaves as an analgesic agent and to elucidate its possible mechanism of antinociceptive action.
Experimental: Phytochemical screening and oral acute toxicity studies were conducted using standard protocols. Antinociceptive potentials were evaluated using acetic acid-induced writhing and hot plate tests in mice. The possible pharmacological mechanism(s) involved in the anti-nociceptive activity were investigated by pretreating mice with Naloxone (2 mg/kg), L-arginine (50 mg/kg), Propranolol (20 mg/kg), Glibenclamide (10 mg/kg), and Prazosin (1 mg/kg)15 min prior to SIL (1000 mg/kg) administration, then assessed using acetic acid-induced writhing 1 h later. Data was analyzed using One-way Anova followed by Bonferroni post hoc test.
Results: Phytochemical screening revealed the presence of alkaloids, flavonoids, saponins, steroids and triterpenes. Oral median lethal dose of SIL was found to be greater than 5000 mg/kg. SIL at the doses of 250, 500 and 1000 mg/kg demonstrated significant (P<0.05) dose-dependent protection against acetic acid-induced writhes in mice. The extract at the highest dose (1000 mg/kg) also significantly (P<0.05) increased the reaction time of mice to thermal stimulus in the hot plate test. Pretreatment with naloxone, prazosin, L-arginine, and propranolol significantly (P<0.05) reduced the antinociceptive activity of the extract. However, pretreatment with glibenclamide showed no effect on its antinociceptive activity.
Recommended applications/industries: The findings of this research has validated the traditional use of the plant in the management of pain through possible involvement of opioidergic, α-adrenergic, β-adrenergic systems including the L-arginine/nitric oxide pathway.
 

کلیدواژه‌ها

Senna italica؛ Antinociception؛ Pain mechanism؛ Adrenergic؛ Opoidergic

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