Molecular Study on the Exon 2 Region of the Ovis Bone Morphology Protein 15 (BMP-15) Gene in Iranian Bluchi Sheep Breed by PCR-SSCP Technique

Iranian Journal of Applied Animal Science

مقاله 13، دوره 4، شماره 4، اسفند 2014، صفحه 769-773   

نوع مقاله: Research/Original Article

نویسندگان

A. Gholibeikifard¹؛ M. Amin Afshar²؛ M. Hosseinpour Mashhadi³؛ H. Mohammadi^{* 4}

¹Department of Animal Science, Technical and Vocational University, Tehran, Iran

²Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

³Department of Animal Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran

⁴Department of Agriculture, Payame Noor University, Tehran, Iran

چکیده

Litter size is one of the most important economical traits in sheep breeding industry. In addition to minor genes, litter size is under the influence of major genes. Bone morphogenetic protein 15 (BMP-15), a member of the transforming growth factor beta (TGF-ß) superfamily, which is specifically expressed in oocytes, plays a dramatic role in sheep prolificacy. Reported mutations in this gene cause increased ovulation rate and infertility in a dosage-sensitive manner. Six different point mutations have been indicated in the BMP-15 gene of sheep, each having a major effect on prolificacy. The aim of the current study was to investigate the Lacaune (FecX^L) mutation in the prolificacy of the Iranian Baluchi sheep breed. Blood samples were collected in ethylenediaminetetraacetic acid(EDTA) coated tubes from jugular vein and genomic DNA was extracted from whole blood samples. Single nucleotide polymorphism of FecX^L loci in BMP-15 gene were determined using polymerase chain reactionsingle-strand conformation polymorphism(PCR-SSCP) technique. No evidence of mutation in FecX^L was observed, all of which were monomorphic for exon 2 BMP-15 gene.

کلیدواژه‌ها

BMP-15؛ FecXL؛ gene؛ litter size؛ polymorphism؛ TGF-ß

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