Chemical constituents, antioxidant and antibacterial activities of the hexane extract of Alchemilla sericata Reichenb

Trends in Phytochemical Research

مقاله 3، دوره 1، شماره 1، خرداد 2017، صفحه 9-14   

نوع مقاله: Original Article

نویسندگان

Ali Shafaghat^{* 1}؛ Akram Panahi²؛ Masoud Shafaghatlonbar³

¹Department of Chemistry, Khalkhal Branch, Islamic Azad University, Khalkhal, Iran

²Department of Chemistry, Ardabil Branch, Islamic Azad University, Ardabil, Iran

³Department of Chemistry, College of Sciences, University of Birjand, Birjand, Iran

چکیده

The hexane extract from the aerial parts of Alchemilla sericata Reichenb. which was collected from Khalkhal-Asalem road, Ardabil Province, northwestern Iran, was obtained using a commercially available Soxhlet apparatus. The fatty acids were derived to methyl esters and determined through gas chromatography/flame ionization detector (GC/FID) and gas chromatography/mass spectrometry (GC/MS) systems. The extract was characterized by high amounts of saturated fatty acids (SFA) (66.0%) along with lower quantities of some other terpenoid compounds. Accordingly, the main components of the hexane extract were found to be hexadecanoic acid (41.4%), 9,12-octadecadienoic acid (21.0%), octadecanoic acid (18.0%) and dioctylphthalate (7.7%), respectively. The hexane extract from A. sericata was also detected as a rich source of an important source of palmitic acid compound. In addition, its antioxidant activity was evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The results indicate that extracts from the aerial parts of A. sericata possess considerable antioxidant activity. The highest radical scavenging activity was detected in this plant oil (IC₅₀=185 μg/mL). The antimicrobial activity of the hexane extract sample were determined against seven Gram-positive and Gram-negative bacteria (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae), as well as three fungi (Candida albicans, Saccharomyces cerevisiae and Aspergillus niger). The bioassays used showed that the hexane extract exhibited a moderate antimicrobial activity. This study revealed that the extracts from the aerial parts of this plant are potential sources of fatty acid components, as well as effective natural antioxidants.

کلیدواژه‌ها

Alchemilla sericata؛ Antioxidant activity؛ Antimicrobial activity؛ Fatty acid؛ Palmitic acid؛ Rosaceae

مراجع

Ayaz, F.A., Hayirlioglu- Ayaz, S., 2001. Total phenols and condensed tannins in the leaves of some Alchemilla species. Biolog. Bratislava. 56, 449-453.

Baron, E.J., Finegold, S.M., 1990. Methods for testing antimicrobial effectiveness. In: Diagnostic Microbiology. Stephanie M (Ed.). Baltimore, Mosby, pp. 171-194.

Buckle, J., 1999. Use of aromatherapy as a complementary treatment for chronic pain. Altern. Ther. Health Med. 5, 42-45.

Chang, S., Abbaspour, H., Nafchi, A.M., Heydari, A., Mohammadhosseini, M., 2016. Iranian Foeniculum vulgare essential oil and alcoholic extracts: Chemical composition, antimicrobial, antioxidant and application in olive oil preservation. J. Essent. Oil-Bear. Plants 19, 1920-1931.

Cheung, L.M., Cheung, P.C.K., Ooi, V.E.C., 2003. Antioxidant activity and total phenolics of edible mushroom extracts. Food Chem. 81, 249-255.

Condrat, D., Mosoarca, C., Zamfir, A.D., Crişan, F., Szabo, M.R., Lupea, A.X., 2010. Qualitative and quantitative analysis of gallic acid in Alchemilla vulgaris, Allium ursinum, Acorus calamus and Solidago virga-aurea by chip-electros pray ionization mass spectrometry and high performance liquid chromatography. Cent. Europ. J. Chem. 8, 530-535.

Felser, C., Schimmer, O., 1999. Two new flavonoid glycosides from the leaves of Alchemilla speciosa. Planta Med. 65(7), 668-670.

Fernandez pachon, M.S., Villano, D., Troncoso, A.M., Garcia parrilla, M.C., 2006. Determination of the phenolic composition of sherry and table white wines by liquid chromatography and their relation with antioxidant activity. Anal. Chim. Acta. 101, 108- 563.

Ivancheva, S., Stantcheva, B., 2000. Ethnobotanical inventory of medicinal plants in Bulgaria. J. Ethnopharm. 165, 69-172.

Karamac, M., Kosinska, A., Pegg, R.B., 2005. Comparison of radical scavenging activities for selected phenolic acids. Polish J. Food. Nut. Sci. 14(2), 165- 170.

Karlova, T., Polakova, L., Smidrkal, J., Filip, V., 2010. Antimicrobial effects of fatty acid fructose esters. Czech J. Food Sci. 28, 146-149.

Mohammadhosseini, M., Akbarzadeh, A., Hashemi-Moghaddam, H., 2016. Gas chromatographic-mass spectrometric analysis of volatiles obtained by HS-SPME-GC-MS technique from Stachys lavandulifolia and evaluation for biological activity: A review. J. Essent. Oil-Bear. Plants 19, 1300-1327.

Mozaffarian, V. 2007. A Dictionary of Iranian Plant Names. Farhang Moaser Publishers, Tehran, Iran.

Nikolova, M., Dincheva, I., Vitkova, A., Badjakov, I., 2012. Phenolic acids and free radical scavenging activity of Alchemilla jumrukczalica Pawl. Inter. J. Pharmac. Sci. Res. 3(3), 802-804.

Olafsdottir, E.S., Omarsdottir, S., Jaroszewski, J.W., 2001. Constituents of three Icelandic Alchemilla species. Biochem. System Ecolog. 29, 959-962.

Shafaghat, A., 2011. Chemical constituents, antimicrobial and antioxidant activity of the hexane extract from root and seed of Levisticum persicum Freyn and Bornm. J. Medic. Plants Res. 5(20), 5127-5131.

Sylvestre, M., Pichette, A., Longtin, A., Nagau, F., Legault, J., 2006. Essential oil analysis and anticancer activity of leaf essential oil of Croton flavens L. from Guadeloupe. J. Ethnopharmacol. 103, 99-102.