ارزیابی و مقایسه میزان فنل، فلاونوئید کل، رزوراترول و ظرفیت آنتی‌اکسیدانی در میوه گونه‌های دارویی (Vitis vinifera)، (vera Pistacia)، (Sambucus nigra) و (Ilex spinigera)

اکوفیتوشیمی گیاهان دارویی

مقاله 4، دوره 5، شماره 2 - شماره پیاپی 18، شهریور 1396، صفحه 37-48   

نوع مقاله: مقاله پژوهشی

نویسندگان

روح اله دستور^{* 1}؛ داود بخشی²؛ علیرضا علی اکبر³

¹دانشجوی دکتری گروه باغبانی، گیاهان دارویی و معطر، پردیس دانشگاهی2، دانشگاه گیلان، رشت، ایران

²دانشیار گروه علوم باغبانی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران

³دانشیار گروه شیمی دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران

چکیده

رزوراترول یک پلی­فنل با ظرفیت آنتی­اکسیدانی بالا است که توانایی مهار یا به تاخیر انداختن طیف گسترده‌ای از بیماری‌ها را دارد. انگور قرمز، پسته، آقطی سیاه وخاس از منابع رزوراترول هستند که در کشور ما به­وفور وجود دارند. به همین منظور، در سال 1395 نمونه‌های گیاهی از 5 منطقه مختلف کشور جمع­آوری گردید و عصاره‌گیری نمونه‌ها با استفاده از روش خیساندن (ماسراسیون) در مرحله رسیدگی کامل میوه  انجام گرفت. سپس میزان فنل، فلاونویید کل، ظرفیت آنتی اکسیدانی و رزوراترول در انگورسیاه رقم شاهانی (Vitis vinifera cv. shahani) و رقم شاهرودی (Vitis vinifera cv. shahrodi)، پسته (vera cv. ohadi Pistacia)، آقطی سیاه (Sambucus nigra) و خاس (Ilex spinigera) به‌ترتیب با روش­های فولین-سیوکالتو، کلریمتری آلومینیوم کلراید و ظرفیت مهار رادیکال­های DPPH و کروماتوگرافی مایع با کارایی بالا (HPLC) اندازه­گیری شد. نتایج نشان داد که بیشترین مقدار فنل در پوست میوه آقطی سیاه و بیشترین مقدار فلاونوئید در پوست انگور شانی مشاهده شد. همچنین، بیشترین ظرفیت آنتی­اکسیدانی در پوست آقطی سیاه و خاس گزارش شد. انگور شانی نیز غنی­ترین منبع رزوراترول بود (003/0±04/0 میلی­گرم بر گرم وزن خشک)، اما آقطی سیاه و دانه پسته اوحدی هم دارای مقادیر قابل توجهی رزوراترول بودند. به­طورکلی، منابع گیاهی مورد بررسی در این تحقیق، قابلیت کاربرد در صنایع دارویی برای استخراج آنتی‌اکسیدان­های طبیعی را دارا هستند.
 

کلیدواژه‌ها

آنتی‌اکسیدان؛ آقطی سیاه؛ انگور؛ پسته؛ خاس؛ فلاونویید؛ فنل و رزوراترول؛ کروماتوگرافی مایع

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