ارزیابی ژنتیکی برخی جمعیت‌های گیاه دارویی زیره‌ سیاه (Carum carvi)با استفاده از نشانگرهای RAPD و ISSR

فیزیولوژی محیطی گیاهی

مقاله 7، دوره 12، شماره 48، دی 1396، صفحه 78-91   

نوع مقاله: پژوهشی

نویسندگان

لیلا فهمیده^{* 1}؛ لیلا جانی پور²؛ بهمن فاضل نسب³

¹گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران

²روه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران

³پژوهشکده کشاورزی دانشگاه زابل، زابل، ایران

چکیده

در این تحقیق به‌منظور، تعیین رابطه خویشاوندی و فاصله ژنتیکی بین جمعیت‌های زیره­ی سیاه از نشانگرهای RAPD و ISSR استفاده شد. با استفاده از نشانگر RAPD در مجموع 126 آلل تکثیر شد که آغازگر TIBMBC08 و TIBMBA02 با 7 آلل کمترین تعداد و آغازگر  TIBMBC05با 22 آلل بیشترین تعداد داشتند. در نشانگر ISSR در مجموع 79 الل شناسایی که آغازگر UBC112 با 1 الل کمترین تعداد و آغازگر UBC809 با 15 آلل بیشترین تعداد داشتند. در نشانگر RAPD، بیشترین میزان شاخص چند شکلی با میزان 94/0 مربوط به آغازگر  TIBMBC05و کمترین میزان شاخص چندشکلی با میزان 8/0 مربوط به آغازگر TIBMBA02 و میزان شاخص تنوع شانن (57/0) و شاخص تنوع نی (38/0) مشاهده گردید. همچنین در نشانگر ISSR، بیشترین میزان شاخص چندشکلی با میزان 92/0 مربوط به آغازگرهای UBC809 و کمترین میزان شاخص چندشکلی مربوط به آغازگر  UBC112با میزان صفر و میزان شاخص تنوع شانن (57/0) و شاخص تنوع نی (39/0) مشاهده گردید. بر اساس اطلاعات RAPD و ISSR به‌صورت توأم بیشترین میزان تشابه مربوط به زیره­ی سیاه کرمان و زیره­ی سیاه جندق (48/0) و کمترین میزان تشابه مربوط به ژنوتیپ­های زیره­ی سیاه کرمان و زیره­ی سیاه نیشابور (115/0) با میانگین 315/0 می­باشد. تجزیه خوشه‌ای جمعیت‌های زیره­ی سیاه را بر اساس اطلاعات توأم هر دو نشانگر RAPD و ISSR در سه گروه مختلف که تا حدودی با موقعیت جغرافیایی آنها مطابفت داشت، طبقه بندی و از همدیگر تفکیک نمود.
 

کلیدواژه‌ها

تنـوع ژنتیکی؛ تجزیه خوشه‌ای؛ زیره‌ سیاه؛ شاخص چندشکلی؛ نشانگر RAPD و ISSR

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