Evaluation of plant pigments existence in medicinal plant, sumac (Rhus coriaria L.) at two different seasons

Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

مقاله 7، دوره 4، شماره 2، مهر 2013، صفحه 95-100   

نوع مقاله: Original article

نویسندگان

Azra Arab¹؛ Majid Talebi^{* 1}؛ Badraldin Ebrahim Sayed Tabatabaei¹؛ Mehdi Rahimmalek²

¹Department of Plant Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan Iran;

²Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan Iran;

چکیده

Background & Aim: The pigments inside the leaf chloroplasts receive sunlight energy and transport it to photosynthesis reaction center. The most important of these pigments is chlorophyll. In this study, the pigments existence in medicinal plant, sumac (Rhus coriaria L.), was investigated at Biotechnology Laboratory, Isfahan University of Technology. Experimental: Experiments were performed in three replicates by using the upper, middle and lower leaves of sumac. Pigments were extracted by 80% acetone from fresh leaves of the sumac. Extracts have been used for the reading at 645, 663 and 470 nm absorption wavelength for chlorophyll a, chlorophyll b and carotenoids, respectively. Results & Discussion: The results showed significant difference between the amounts of pigments in different seasons (p< 0.05). The amount of chlorophyll a, b, total chlorophyll and carotenoids was obtained in spring on average 0.78, 0.24, 1.02 and 3.32 mg/g of leaf, whereas it was 1.55, 0.46, 2.01 and 6.61 mg/g of leaf for winter, respectively. The total amount of carotenoid was higher than chlorophylls and both of them were high in early winter. Totally, chlorophyll a content was higher than chlorophyll b in both seasons, as well as other plants. Recommended applications/industries: Chlorophyll, carotenoids and their derivatives are important antioxidants that there are plenty of them in sumac, it reveals the potential for its use in the food industry.

کلیدواژه‌ها

Rhus coriaria؛ Pigment؛ Photosynthesis

مراجع

Alan Blackburn, G. 2007. Hyperspectral remote sensing of plant pigments.  Journal of Experimental Botany., 58: 855–867.

Abdirad, S., Rezanejad, F. and Kalantari, K. F. 2011. The effect of different light intensities on callogenesis and calli pigments content of shoot and floral explants of Rosa damascena Mill. and Rosa miniature. Journal of Agricultural Biotechnology., 3: 43-65.

Arnon D. T. 1949. Copper enzymes in isolation chloroplast phenoloxidase in Beta vulgaris. Plant Physiology., 24: 1- 15.

Connell, S. L. and Al-Hamdani, S. H. 2001. Selected physiological responses of kudzu to different chromium concentrations. Canadian Journal of Plant Science., 81:33-58.

Dere, S., Gunes, T. and Sivaci, R. 1998. Spectrophotometric determination of chlorophyll - a, b and total carotenoid contents of some algae species using different solvents. Turkish Journal of Botany., 22: 13-17.

Dorudi, H., Akbarinia, M. Jalali, S.G. and Khosrowgerdi, A. 2008. Effects of cutting diameter and media on rooting and survival of sumac cutting (Rhus coriaria L.). Iranian Journal of Biology., 2: 1-7.

Heidari Sharif-Abad, H. 2001. Plants and Salinity. Research Institute of Forests and Rangelands Press.

Hopkins, W. J. Ahmadi, A. Ehsanzadeh, P. and Jabari, F. 2004. Introduction to Plant Physiology, Tehran University. Institute of Publishing and Printing.

Javaheri, SH., Abdollahian-Noghabi, Kashani, M. A. Noshad, H. and Habibi, D. 2011. Effect of leaf position and age on the nitrogen content and chlorophyll meter values in sugar beet. Iranian Journal of Field Crop Science., 13: 87-98.

Kadam, V. B., Wadikar, M. S. and Ahire,  P. P. 2008. Bio-chemical analysis of leaves of some medicinal plants of Laling forest. Dhule District (M.S.), India. Journal Plant Archives., 8:293-294.

Kawamitsu, Y. Sinh, R. K., Nelson, B. J., Tamaki, Y. and Murayama, S. 1999. Effects of nitrogen supply growth characteristics and leaf photosynthesis in sugarcane. Faculty of Agriculture, University of the Ryukys., 46: 1-14.

Lahooti, M., 1988. Principles of Plant Physiology. Publisher by Astan Quds Razavi, Mashhad, Iran.

Masuda, T., Tanaka, A. and Melis, A. 2002. Chlorophyll antenna size adjustments by irradiance in Dunaliella salina involve coordinate regulation of chlorophyll a oxygenase (CAO) and Lhcb gene expression. U.S. DOE Hydrogen Program Review., 1-22.

Misyura, M., Colasanti, J. and Rothstein, S. J. 2012. Physiological and genetic analysis of Arabidopsis thaliana anthocyanin biosynthesis mutants under chronic adyerse environmental conditions. Journal of Experimental Botany. 2-12.,

Mlodzinska, E. 2009. Survey of plant pigments: molecular and environmental determinants of plant. Acta Biologica Cracoviensia Series Botanica., 51: 7–16.

Mohammadi, M. Habibi, D. Ardakani, M. R. and Asgharzadeh, A. 2009. Effect of biological fertilizers, humic acid and superabsorbent polymer on chlorophyll content, lipid membrane and activity of superoxide dismutase and catalase enzymes in annual medic (Medicago scutellata) under cadmium toxicity. Agronomy and Plant Breeding Journal., 6(2):65-79.

Mozaffar, A. 1974. Plant Physiology. University Press of Agriculture and Animal Husbandry of University.

Ozcan, M. and Haciseferogullari, H. 2004. A condiment [sumac (Rhus coriaria L.) fruits]:some physico-chemical properties. Bulgarian Journal of Plant Physiology., 30: 74-84.

Rawashdeh, I. M., Ghzawi, A. L.,  Rawashdeh, N. Q., Khairallh, K., Al-Tawaha, A. R. and Salama, B. 2009. Genetic variation among sumac (Rhus Coriaria L.) samples collected from three locations in Jordan as revealed by AFLP markers. Advances in Environmental Biology., 3: 107-112.

Stuart, V., Sathyendranath, S., Platt, T., Maass, H. and D.Irwin, B. 1998. Pigments and species composition of natural phytoplankton populations: effect on the absorption spectra. Journal of Plankton Research., 20: 187-217.

Ustin, S., Gitelson, A. A., Jacquemoud, S., Schaepman, M., Asner, G., Gamon, J. and Zarco-Tejada, P. 2009. Retrieval of foliar information about plant pigment systems from high resolution spectroscopy. Remote Sensing of Environment., 113: 67–77.

Yanagi, K. and Koyama, T. 1971. Thin layer chromatographic method for determining plant pigments in marine particulate matter, and ecological significance of the results. Geochemical Journal., 5: 23–37.

Zahedi, A. 1967. Photosynthesis. Tehran University Press.

Zaker, A., Lahouti, M. Abrishamchi, P. and Ejtehadi, H. 2005. Study on the effects of Cr⁺³ and Cr⁺⁶ accumulation on growth and chlorophyll content in parsley (Petroselinum crispum). Iranian Journal of Biology., 18:101-109.