Chemical composition and antimicrobial activity of essential oil of Salvia officinalis L. and Salvia virgata Jacq

Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

مقاله 1، دوره 8، شماره 2، آبان 2017، صفحه 71-78   

نوع مقاله: Original article

شناسه دیجیتال (DOI): 10.18869/JHD.2017.71

نویسندگان

Ahmad Reza Golparvar^{* 1}؛ Amin Hadipanah²؛ Mohammad Mehdi Gheisari³؛ Davood Naderi⁴؛ Shima Rahmaniyan⁵؛ Mojtaba Khorrami⁶

¹Department of Agronomy and plant Breeding, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

²Department of Horticultural Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

³Toxicology Research Center, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

⁴Department of Horticulture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

⁵Department of Horticultural Sciences, Yasouj Branch, Islamic Azad University, Yasouj, Iran

⁶Department of Horticultural Sciences, Hormozgan University, Bandar Abbas, Iran

چکیده

Background & Aim:The genus Salvia (commonly known as sage) is a broad genus belonging to the family Lamiaceae. This study is aimed at assessing the essential oil composition and antimicrobial activities of two Salvia species.
Experimental: The chemical composition of the essential oils of Salvia officinalis L. and Salvia virgata Jacq, cultivated in Estahban (Fars, South Iran), was studied by means of GC-MSanalyses. Antimicrobial activity was tested against a panel of microorganisms including one Gram-positive (Staphylococcus epidermidis PTCC NO. 1435) and one Gram-negative (Escherichia coli) and three fungal strain (Alternaria alternata PTCC NO. 5224, Penicillium funiculosum PTCC NO. 5301 and P. funiculosum PTCC NO. 5169) using the disk diffusion and agar-well diffusion methods and the Minimum Inhibitory Concentration (MIC) technique.
Results: In all, 57 compounds were identified, 42 for S. officinalis, accounting for 98.94% ofthe total oil, 29 for S. virgata (98.81%). The major components of S. officinalis essential oil were α-thujone (37.18%), 1,8-cineole (12.71%), β-thujone (9.10%) and the major components of S. virgata essential oil were caryophyllene oxide (30.23%), β-caryophyllene (22.63%), sabinene (11.82%). The antimicrobial activity of the total essential oil evaluated by the agar-well diffusion method, the results showed that the highest active against S. epidermidis and also the least active against E. coli. Inhibition of growth was tested by the disk diffusion method, the results showed that essential oil of S. officinalis and S. virgata were highest active against E. coli and P. funiculosum (PTCC NO. 5301), respectively. Also the least active against A. alternata.
Recommended applications/industries: The results showed thatS. officinalis oil had higher antimicrobial activity compare to S. virgata

کلیدواژه‌ها

Salvia officinalis L؛ Salvia virgata Jacq؛ Chemical constitutes؛ Antimicrobial Activity

مراجع

Adams, R.P. 2007. Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometery, 4th edition (Allured Publishing Corporation, Carol Stream, IL) 456.

Akin, M., Demirci, B., Bagci, Y. and Baser, K.H.C. 2010. Antibacterial activity and composition of the essential oils of two endemic Salvia sp. from Turkey. African Journal of Biotechnology, 9: 2322-2327.

Bachir Raho, G., Benattouche, Z., Bevilacqua, A., Corbo, M.R., Sinigaglia, M. and Pignatiello, S. 2016. Antimicrobial Activity of Extracts from Salvia officinalis L on some Bacteria and Yeast. Journal of Biotechnology Research, 2(5): 31-35.

British Pharmacopoeia. 1988. British pharmacopoeia, vol. 2. HMSO, London, pp. 137–138.

Carson, C.F., Mee, B.J. and Riley, T.V. 2002. Mechanism of action of Melaleuca alternifolia (tea tree) oil on Staphylococcus aureus determined by time-kill, lysis, leakage and salt tolerance assays and electron microscopy. Antimicrobial Agents and Chemotherapy, 46: 1914-1920.

Ceylan, E. and Fung, D.Y.C. 2004. Antimicrobial Activity of Spices. Journal of Rapid Methods and Automation in Microbiology, 12: 1-55.

Delamare, A.P.L. 2007. Antibacterial activity of the essential oils of Salvia officinalis L. and Salvia triloba L. cultivated in South Brazil. Food Chemistry, 100. 603-608.

Farhat, M.B., Jordan, M.J. and Chaouech-Hamada, R. 2009. Variations in essential oil, phenolic compounds, and antioxidant activity of Tunisian cultivated Salvia officinalis L. Journal of Agricultural and Food Chemistry, 57: 10349-10356.

Fellah, S., Diouf, P.N. and Petrissans, M. 2006. Chemical composition and antioxidant properties of Salvia officinalis L. oil from two culture sites in Tunisia. Journal of Essential Oil Research, 18(5): 553-556.

Friedman, M., Henika, R.P. and Mandrell, E.R. 2002. Bactericidal activities of plant essential oils and some of their isolated constituents against Campylobacter jejuni, Escherichia coli, Listeria monocytogenes, and Salmonella enterica. Journal of Food Protection, 65: 1545-1560.

Ghasemi Pirbalouti, A., Jahanbazi, P., Enteshari, S., Malekpoor, F. and Hamedi, B. 2010. Antimicrobial activity of some Iranian medicinal plants. Archives of Biological Science Belgrade, 62(3): 633-642.

Ghasemi Pirbalouti, A., Taheri, M., Raisee, M., Bahrami, H.R. and Abdizadeh, R. 2009. In vitro antifungal activity of plant extracts on Saprolegnia parasitica from cutaneous lesions of rainbow trout (Oncorhynchus mykiss) eggs. The Journal of Food, Agriculture & Environment, 7: 94-96.

Golparvar A.R. and Hadipanah, A. 2013. Identification of the components of sage (Salvia officinalis L.) and thyme (Thymus vulgaris L.) cultivated in Isfahan climatic conditions. Electronic Journal of Biology, 9(2): 42-45.

Hadipanah, A. and Khorrami, M. 2016. Chemical composition and antibacterial activity of essential oil from two Thymus species. Journal of Herbal Drugs, 6(4): 211-217.

Hara-Kudo, Y., Kobayashi, A., Sugita-Konishi, Y. and Kondo, K. 2004. Antibacterial activity of plants used in cooking for aroma and taste. Journal of Food Protection,67: 2820–2824.

Javidnia, K., Miri, R., Soltani, M., Gholami, M. and Khosravi, A. 2008. Antimicrobial activity and chemical composition of the essential oils of six Iranian Salvia species. Chemistry of Natural Compounds, 44(5): 654–658.

Klaus, A., Beatovic, D., Niksic, M. Jelacic, S. and Petrovic, T. 2009. Antibacterial activity of aromatic plants essential oils from Serbia against the Listeria monocytogenes. Journal of Agricultural Sciences, 54(2): 95-104.

Kozlowska, M., Laudy A.E., Przybyl, J., Ziarno, M. and Majewska, E. 2015. Chmical composition and antibacterial activity of some medicinal plants from Lamiaceae family. Acta Poloniae Pharmaceutica-Drug Research, 72(4): 757-767.

Lu, Y. and Foo, L.Y. 2002. Polyphenolics of Salvia. A review. Phytochemistry, 59: 114-140.

Naghibi, F., Mosaddegh, M., Mohammadi Motamed, M. and Ghorbani, A. 2010. Labiatae family in folk medicine in Iran: From ethnobotany to pharmacology. Iranian Journal of Pharmaceutical Research, 4: 63-79.

Raut, J.S. and Karuppayil, S.M. 2014. A status review on the medicinal properties of essential oils, Industrial Crops and Products, 62: 250–264.

Said-Al Ahl, H., Hussein, M.S., Gendy, A.S.H. and Tkachenko, K. 2015. Quality of sage (Salvia officinalis L.) Essential OilGrown in Egypt.International Journal of Plant Science and Ecology, 1(4): 119-123.

Sefidkon, F. and Mirza, M. 1999. Chemical composition of the essential oils of two Salvia species from Iran, Salvia virgata Jacq. and Salvia syriaca L. Flavour and Fragrance Journal, 14(1): 45-46.

Skaltsa, H.D., Demetzos, C., Lazari, D. and Sokovic, M. 2003. Essential oil analysis and antimicrobial activity of eight Stachys species from Greece. Phytochemistry, 64(3): 743-752.

Sokovic, M., Glamoclija, J., Marin, P.D., Brkic, D. and Van Griensven, L.J.L. 2010. Antibacterial effects of the essential oils of commonly consumed medicinal herbs using an In Vitro model. Molecules, 15: 7532-7546.

Tenore, G.C., Ciampaglia, R., Arnold, N.A., Piozzi, F., Napolitano, F., Rigano, D. and Senatore, F. 2010. Antimicrobial and antioxidant properties of the essential oil of Salvia lanigera from Cyprus. Food and Chemical Toxicology, 49(1): 238-243.

Velickovic, D., Randjelovic, N. and Ristic, M. 2003. Chemical constituents and antimicrobial activity of the ethanol extracts obtained from the flower, leaf and stem of Salvia officinalis L. Journal of the Serbian Chemical Society, 68(1): 17-24.

Wise, M.L., Savage, T.J., Katahira, E. and Croteau, R. 1998. Monoterpene synthases from common sage (Salvia officinalis). cDNA isolation, characterization, and functional expression of (+)-sabinene synthase, 1,8-cineole synthase, and (+)-bornyl diphosphate synthase. Journal of Biological Chemistry, 273(24): 14891-14899.