Hepatoprotective Properties of Ethanol Seed Extract of Citrus paradisi Macfad (Grape Fruit) Against Paracetamol-Induced Hepatotoxicity in Wistar Rats

Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

مقاله 3، دوره 8، شماره 4، اردیبهشت 2018، صفحه 219-225   

نوع مقاله: Original article

شناسه دیجیتال (DOI): 10.14196/JHD. 2018. 219

نویسندگان

Godswill J Udom^{* 1، 2}؛ Omoniyi K Yemitan³؛ Emem E Umoh¹؛ H. O. C Mbagwu¹؛ Ekemini E. Ukpe¹؛ Paul S. Thomas⁴

¹Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, Uyo, Nigeria

²Gimmex Health Consult, Suites B6 Real Towers Complex, 26 Ekukinam Street, Utako District, Abuja, Nigeria

³Department of Pharmacology, Therapeutics and Toxicology, Lagos State University College of Medicine, Lagos, Nigeria

⁴Department of Pharmacognosy and Natural Medicine, Faculty of Pharmacy, University of Uyo, Uyo, Nigeria

چکیده

Background & Aim: The human body has the liver as one of its largest organs. It serves as the major site for metabolism and excretion. Injury to the liver or impairment of its functions may complicate one’s health and therefore, constitutes one of the serious public health challenges. The ethanol seed extract of Citrus paradisi Macfad (CPE) was carried out to evaluate its protective usefulness on the liver against paracematol-induced liver injury.
Experimental: Thirty adult male Wistar rats were randomly allotted to five groups (6/group) and orally-treated daily with 100 mg/kg body weight of silymarin (positive Control), 10 ml/kg body weight of distilled water (negative control) and CPE (200, 400 and 600 mg/kg) body weight, respectively for 7 days. On the eighth day, all groups were administered 2 g/kg body weight of paracetamol. 24 h thereafter, animals were sacrificed under diethyl ether anesthesia and blood samples were collected by cardiac puncture for biochemical and haematological investigations.
Results: Compared to the negative control, extract (200 – 600 mg/kg) significantly (p<0.05)reduced the activities of ALP, ALT and AST dose-dependently. Extract significantly (p<0.05) elevated all blood parameters except for neutrophil differentials.
Recommended applications/industries: Grapefruit seed extract possesses hepatoprotective potential and can be used as an antidote against paracetamol-induced hepatotoxicity.

کلیدواژه‌ها

Citrus paradisi؛ Hepatocytes؛ Paracetamol-induced hepatotoxicity؛ Hepatoprotection

مراجع

Adeneye, A. A. 2008. Hematopoetic Effect of Methanol Seed Extract of Citrus paradisi Macfad (grapefruit) in Wistar Rats. Biomedical Research, 19(1): 23 – 26.

Ahsan, M. R., Islam, K. M. and Bulbul, I. J. 2009. Hepatoprotective Activity of Methanol Extract of some medicinal plants against Carbon Tetrachloride-induced Hepatotoxicity in Rats. Global Journal of Pharmacology, 3, 116 – 122

Bessems, J. G. M. and Vermeulen, N. P. E. 2001. Paracetamol (acetaminophen)-induced toxicity: Molecular and Biochemical Mechanisms, Analogues and Protective Approaches. Critical Reviews in Toxicology, 31: 55 – 138.

Bessey, O. A., Lowry, O. H. and Brock, M. J. 1946. A Method for the Rapid Determination of Alkaline Phosphate with Few Cubic Millimeters of Serum. Journal of Biological Chemistry; 164, 321 – 329.

Carrington, S., Fraser, and Henry, C. 2003. "Grapefruit". A~Z of Barbados Heritage. Macmillan Caribbean. pp. 90 – 91.

Dahlin, D. C., Miwa, G. T., Lu, A. Y. and Nelson, S. D. 1984. N-acetyl-p-benzoquinone imine: A Cytochrome P-450 Mediated Oxidation Product of Acetaminophen. Proceedings of the National Academy of Science, USA. 81: 1327 – 1331.

Dobbs, N. A., Twelves, C. J, Gregory, W., Cruickshanka, C., Richards, M. A. and Rubens R. D. 2003. Epirubicin in patients with liver dysfunction. Development and evaluation of a novel dose modification scheme. European Journal of Cancer, 39; 580 – 586.

Gupta, V., Kohli, K., Ghaiye, P., Bansal, P. and Lather, A. 2011. Pharmacological potentials of citrus paradisi- an overview. International Journal of Phytotherapy Research, 1(1): 8 – 17

Gupta, M., Dalia, B. and Arup, M. 2013. Studies of anti-inflammatory, anti-pyretic and analgesic effects of aqueous extract of traditional herbal drug on rodents. International Research Journal of Pharmacy, 4(3); 113 – 120.

Henderson, C. J., Wolf, C. R., Kitteringham, N., Powell, H., Otto, D. and Park, B. K. 2000. Increased Resistance to Acetaminophen Hepatotoxicity in Mice Lacking Glutathione S-transferase. Proceedings of the. National. Academy of Science, USA. 97: 12741 – 12745.

Ionescu, G., Kiehl, R., Wichmann-Kunz, F., Williams, C., Ba, L. and Levine, S. 1990. Oral Citrus Seed Extract in Atopic Eczema: In Vitro and In Vivo Studies on Intestinal Microflora. Journal of Orthomolecular Medicine, 5(3): 155 – 7.

James, L. P,. Mayeux, P. R. and Hinson, J. A. 2003. Acetaminophen-induced Hepatotoxicity. Drug Metabolism and Disposition, 31(12): 1499–1506.

Marcus, R. and Coulston, A. M. 2001. Water-soluble vitamins: the Vitamin B Complex and Ascorbic Acid. In: Goodman and Gilman’s, The Pharmacological Basis of Therapeutics (Hardman JG and Limbird LE eds.). 10^th ed., New York: McGraw-Hill Medical Publishing Division, pp. 1753-1791

NIH [National Institutes of Health, 1996. Revised Guide for the Care and Use of Laboratory Animals. In: NIH Guide, 25, 28.

OECD, 2001. Acute Oral Toxicity (AOT, Test Guideline 425) Statistical Programme (AOT425StatPPgm), version 1.0. Available online at http://www. oecd. org/ OECD/ pages/home/displaygeneral/ 0,3380, EN-document-524-nodirectorate-no-24-6775-8,FF.html

Osilesi, O., Adeiyi, A., Ogunyemi, E. O. and Fakunle, J. B. 1997. The glycaemic response to selected fruits and vegetables in Nigerian diabetics. African Journal of Medicine and Pharmaceutical Sciences, 1; 1-6

Ramalakshmi, K., Rahath, K. I., Rao, L. J. 2007. Antioxidant potential of low-grade coffee beans. Journal of Food Science, 41; 96–103.

Reid, A. B., Kurten, R. C., McCullough, S. S, Brock, R. W. and Hinson, J. A. 2005. Mechanisms of Acetaminophen induced hepatotoxicity: Role of Oxidative Stress and Mitochondrial Permeability Transition in freshly Isolated Muse Hepatocytes. Journal of Pharmacology and Experimental Therapeutics, 312; 509 – 516.

Reitman, S. and Frankel, S. 1957. Glutamic-pryuvate Transaminase Assay by Colorimetric Method. American Journal of Clinical Pathology, 28, 56.

Sofowora, A. 1993. Medicinal plants and Traditional Medicine in Africa. 2^nd ed., Ibadan, Spectrum Ltd., p.152.

Stickel, F. and Schuppan, D. 2007. Herbal Medicine in the Treatment of Liver Diseases. Digestive and Liver Disease, 39; 293–304.

Subramaniam, S., Khan, H. B. H., Elumalai, N. and Lakshmi S. Y. S. 2015. Hepatoprotective Effect of Ethanolic Extract of Whole Plant of Andrographis paniculata against CCl₄–induced Hepatotoxicity in Rats. Comparative Clinical Pathology, 24; 1–7.

Trease, G. E. and Evans, W. C. 1989. A Textbook of Pharmacognosy. 11^th ed., London: Balliere Tindal and Cox, p.475.