اثر محلول پاشی اسید‌آبسیزیک بر ویژگی‌های مورفوفیزیولوژیکی و برخی خصوصیات بیوشیمیایی گیاه .Cannabis sativa L تحت شرایط رطوبتی مختلف خاک

اکوفیتوشیمی گیاهان دارویی

مقاله 2، دوره 7، شماره 1 - شماره پیاپی 25، خرداد 1398، صفحه 12-24   

نوع مقاله: مقاله پژوهشی

نویسندگان

هاجر معتمدی شارک¹؛ خدایار همتی^{* 2}؛ سارا خراسانی نژاد³

¹گروه علوم باغبانی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان

²هیات علمی دانشگاه

³استادیار و عضوهیات علمی دانشگاه علوم کشاورزی و منابع طبیعی گرگان

چکیده

به‌منظور بررسی اثر تنش خشکی و محلول‌پاشی اسیدآبسیزیک بر برخی خصوصیات مورفوفیزیولوژیکی و بیوشیمیایی گیاه دارویی شاهدانه Cannabis sativa L.)) آزمایشی در قالب فاکتوریل بر پایه طرح کاملا تصادفی در مزرعه دانشگاه علوم کشاورزی و منابع طبیعی گرگان در سال زراعی 97-1396 انجام شد. تیمارهای مورد بررسی شامل چهار سطح تنش خشکی (55، 70، 85 و 100 درصد ظرفیت زراعی) و تیمار محلول‌پاشی اسیدآبسیزیک (0، 10، 20، 30 میلی‌گرم بر لیتر) بود. در زمان گلدهی کامل، ویژگی‌های رشدی و مورفولوژی از جمله قطر ساقه، وزن تر و خشک اندام هوایی، وزن تر و خشک ریشه، ارتفاع ساقه، طول ریشه، سطح برگ، تعداد برگ، تعداد بذر، تعداد گل، وزن کل بذور، تعداد شاخه فرعی، قطر شاخه فرعی، فنل کل ریشه، فلاونوئید کل ریشه، فعالیت آنتی‌اکسیدانی ریشه، پرولین ریشه، قندهای محلول برگ و مقدار اسیدآبسیزیک برگ ارزیابی شد. صفات فیزیولوژیکی با استفاده از روش اسپکتروفتومتری و مقدار اسیدآبسیزیک با دستگاه HPLC آنالیز گردید. نتایج نشان داد تنش کم‌آبی و اسیدآبسیزیک اثر معنی‌داری بر تمام صفات مورد بررسی به جزء مقدار اسیدآبسیزیک برگ، تعداد شاخه فرعی و قند کل داشت و بیشترین فنل کل در سطح توام تنش 85 درصد و 30 میلی‌گرم در لیتر اسیدآبسیزیک ثبت شد که اختلاف معنی‌داری بیبر اساس نتایج این پژوهش می‌توان نتیجه گرفت، تنش کم‌آبی، اثرات منفی بر رشد گیاه داشته و در مقابل گیاه برای مقابله با این اثرات ترکیبات فلاونوئیدی و آنتی‌اکسیدانی خود را افزایش داد. محلول‌پاشی اسیدآبسیزیک باعث بهبود برخی صفات در شرایط تنش خشکی شده است.

کلیدواژه‌ها

پرولین؛ فعالیت آنتی‌اکسیدانی؛ فنل کل؛ قندهای محلول؛ مورفولوژیک

مراجع

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