α-Melanocyte-stimulating hormone directly increases the plasma calcitonin level and involves calcium metabolism in goldfish

International Aquatic Research

مقاله 7، دوره 10، شماره 3، آذر 2018، صفحه 283-292   

نوع مقاله: Original research

شناسه دیجیتال (DOI): 10.1007/s40071-018-0206-5

نویسندگان

Takahiro Ikari¹؛ Yuki Kobayashi²؛ Yoichiro Kitani¹؛ Toshio Sekiguchi¹؛ Masato Endo³؛ Akira Kambegawa⁴؛ Kiyoshi Asahina⁵؛ Atsuhiko Hattori⁶؛ Yoshiaki Tabuchi⁷؛ Thumronk Amornsakun⁸؛ Kanta Mizusawa⁹؛ Akiyoshi Takahashi⁹؛ Nobuo Suzuki^{* 1}

¹Noto Marine Laboratory, Division of Marine Environmental Studies, Institute of Nature and Environmental Technology, Kanazawa University, Noto-Cho, Ishikawa, 927-0553, Japan

²Graduate School of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima, 739-8521, Hiroshima, Japan

³Department of Marine Biosciences, Division of Marine Science, Tokyo University of Marine Science and Technology, Minato-Ku, Tokyo, 108-8477, Japan

⁴The Kambegawa Laboratory, Komae, Tokyo, 201-0013, Japan

⁵Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, 252-0813, Kanagawa, Japan

⁶Department of Biology, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, Ichikawa, Chiba, 272-0827, Japan

⁷Division of Molecular Genetics Research, Life Science Research Center, University of Toyama, Toyama, 930-0194, Japan

⁸Fisheries Technology Program, Faculty of Science and Technology, Prince of Songkla University, Pattani, 94000, Pattani, Thailand

⁹School of Marine Biosciences, Kitasato University, Sagamihara, Kanagawa, 252-0373, Japan

چکیده

The effects of α-melanocyte-stimulating hormone (α-MSH) on calcium metabolism were examined with goldfish. The scales on the left side of goldfish bodies were removed to allow the regeneration of scales under anesthesia. Thereafter, the influences of α-MSH injection (low dose: 0.1 μg/g body weight; high dose: 1 μg/g body weight) on plasma calcitonin (calcium-regulating hormone) and the calcium content of the scales were investigated. Ten days after removing the scales, we measured the plasma calcitonin and calcium content of both regenerating scales on the left side and ontogenic scales on the right side. At both doses of α-MSH injection, plasma calcitonin concentrations in the α-MSH-treated group were significantly higher than those in the control group. The mRNA expressions of α-MSH-receptors were detected in the ultimobranchial glands (secretory organ of calcitonin), indicating that α-MSH directly functions in ultimobranchial glands and promotes calcitonin secretion. Furthermore, we found that the calcium content of regenerating scales in α-MSH-treated goldfish was higher than that in control goldfish, while the calcium content of ontogenic scales on the right side was significantly decreased by α-MSH injection. There was a significant co-relationship between plasma calcitonin and the calcium content of regenerating scales. The mRNA expression of calcitonin receptors in regenerating scales was remarkably higher than that in ontogenic scales. These results imply that calcitonin functions to promote scale regeneration resulting from the inhibition of bone resorption because calcitonin suppresses osteoclastic activity. Thus, we are the first to demonstrate the interaction between α-MSH and calcitonin in teleosts.

کلیدواژه‌ها

a-Melanocyte-stimulating hormone؛ Calcitonin؛ Calcium؛ Fish scales؛ Ultimobranchial glands؛ Scale regeneration