اثر ضدباکتریایی نانوذره آلومینات روی بر باکتری‌های بیماری‌زای‌ غذایی اشریشیا کولای و سودوموناس آئروژینوزا

بهداشت مواد غذایی

دوره 10، 2 (38) تابستان، تیر 1399، صفحه 95-108   

نوع مقاله: مقاله پژوهشی

شناسه دیجیتال (DOI): 10.30495/jfh.2020.1885047.1250

نویسندگان

علی طاهری^{* 1}؛ مرتضی ضیاالدینی²؛ معصومه گهرام زئی³

¹دانشیار فرآوری محصولات شیلاتی، دانشکده علوم دریایی، دانشگاه دریانوردی و علوم دریایی چابهار، چابهار، ایران

²استادیار شیمی آلی، دانشکده علوم دریایی، دانشگاه دریانوردی و علوم دریایی چابهار، چابهار، ایران

³دانشجوی کارشناسی ارشد فرآوری محصولات شیلاتی، دانشکده علوم دریایی، دانشگاه دریانوردی و علوم دریایی چابهار، چابهار، ایران

چکیده

مواد در ابعاد نانو که از نسبت سطح به حجم بالایی برخوردارند واکنش‌پذیری بهتری با سایر ترکیبات دارند. گسترش فناوری نانو در دهه‌ی اخیر، فرصت‌هایی برای کشف اثرات ضد باکتریایی نانو ذرات ایجاد کرده است. در این پژوهش خواص ضد باکتریایی نانوذره روی آلومینات(ZnAl₂O₄) علیه باکتری‌های عامل بیماری‌های غذازاد  اشریشیا کولای و سودوموناسآئروژینوزا بررسی شد. با استفاده از نانوذره روی آلومینات سنتز شده فعالیت ضد باکتری به روش انتشار دیسک بر آگار مورد ارزیابی قرار گرفت. قطر هاله‌های عدم رشد نشان‌دهنده میزان حساسیت باکتری‌ها به نانو مواد مورداستفاده بود. حداقل غلظت مهارکنندگی از رشد باکتری‌ها (MIC) و حداقل غلظت کشندگی باکتری‌ها (MBC) به روش ماکرودایلوشن در لوله‌های استریل آزمایشگاهی انجام گرفت. بررسی آماری با آزمون T test انجام گرفت. با استفاده از نتایج به‌دست‌آمده در این بررسی قطر هاله عدم رشد سودوموناس آئروژینوزا (8/1 ± 06/16 میلی­متر) نسبت به اشریشیاکولای (2/2±64/11 میلی­متر) بیشتر بود (p < 0.05). کمترین غلظت مهارکننده رشد و غلظت کشندگی باکتری‌ها مربوط به باکتریسودوموناس آئروژینوزا بود و باکتری اشرشیاکولای فاقد آن بود. با توجه به نتایج به‌دست‌آمده در این مطالعه نانو ذرات روی آلومینات علیه باکتریسودوموناس آئروژینوزا فعالیت ضد باکتری بسیار خوبی نشان داد و می‌تواند به‌عنوان یک ترکیب ضدباکتریایی جدید در مطالعات بسته‌بندی مواد غذایی مورداستفاده قرار گیرد.

کلیدواژه‌ها

خاصیت ضدباکتریایی؛ نانوذره؛ آلومینات روی؛ اشریشیا کولای؛ سودوموناس آئروژینوز

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