اثر غربالگری و بهینه‌سازی اسموپرایمینگ بر خصوصیات جوانه‌زنی بذور گندم (.Triticum aestivum L) با استفاده از روش سطح- پاسخ

فیزیولوژی محیطی گیاهی

دوره 17، شماره 67، مهر 1401، صفحه 23-37   

نوع مقاله: پژوهشی

شناسه دیجیتال (DOI): 10.30495/iper.2021.1937928.1719

نویسندگان

آفاق یاوری^* ؛ قادر حبیبی؛ معصومه عابدینی؛ غلامرضا بخشی خانیکی

گروه زیست شناسی، دانشگاه پیام نور، تهران، ایران

چکیده

اسموپرایمینگ بذر یک استراتژی موثر برای بهبود فرایند جوانه­زنی است. غربالگری و برآورد غلظت­های بهینه­ ترکیبات مختلف اسمزی اولین قدم در اسموپرایمینگ دانه محسوب می­شود. دراین تحقیق ابتدا ارزیابی پرایمینگ تک­تیماری غلظت­های مختلف کلرید سدیم (0، 1، 2، 3، 4، 5، 10، 20، 30 گرم بر لیتر)، سولفات روی (0، 1، 5، 10، 20، 30 و 40 میلی­مولار)، پرولین (0، 1، 5، 10، 20، 30 و 40 میلی­مولار) و ترهالوز (0، 1، 5، 10، 20، 30 و 40 میلی‌مولار) بر روی بذور گندم انجام و سپس روش سطح- پاسخ در قالب طرح باکس- بنکن طراحی شد. نتایج حاصل نشان­دهنده افزایش معنی­دار جوانه­زنی گندم در غلظت­های 3 و 10 گرم بر  لیتر کلرید سدیم، 1 و 20 میلی­مولار سولفات روی، 1 و 10 میلی­مولار پرولین و 0 و 1 میلی­مولار ترهالوز بود. این غلظت‌ها با استفاده از روش سطح- پاسخ و با انجام آزمون­های آزمایشگاهی اعتبارسنجی شدند. مقادیر ضریب تبیین (99/0=R²) و R² تصحیح شده (99/0₌R²_adj) نشان داد که مدل به‌دست آمده برای تحلیل داده­ها مناسب می­باشد. آنالیز واریانس یک طرفه (0001/0>P) نشان داد که مدل درجه دوم بهترین مدل برای تعیین برهمکنش متغیرهای مورد مطالعه می­باشد و مقادیر بهینه شامل کلرید سدیم 3 گرم بر لیتر، سولفات روی 1 میلی­مولار و پرولین 1 میلی­مولار به‌صورت ترکیبی پیشنهاد شد. نتایج آزمایش تکمیلی با استفاده از مقادیر پیش­بینی شده توسط مدل، مشخص ساخت که روش سطح- پاسخ می­تواند در آزمون­های غربالگری و  بهینه­سازی جوانه­زنی مورد استفاده قرار گیرد.

کلیدواژه‌ها

ترکیبات اسمزی؛ طراحی آزمایش؛ طرح باکس- بنکن؛ عملکرد بذر؛ مدل سازی

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