اثر کلرید سدیم بر خصوصیات فیزیولوژیکی، بیوشیمیایی و بیان دو ژنADS و CYP71AV1دخیل در مسیر بیوسنتزی آرتمیزینین در گیاه افسنطین (Artemisia absinthium)

فیزیولوژی محیطی گیاهی

دوره 17، شماره 67، مهر 1401، صفحه 38-60   

نوع مقاله: پژوهشی

شناسه دیجیتال (DOI): 10.30495/iper.2022.690244

نویسندگان

سارا سلیمیان ریزی¹؛ زهرا رضایتمند^{* 1}؛ منیره رنجبر¹؛ نسرین یزدان پناهی²؛ زرین دخت امامی³

¹گروه زیست شناسی، واحد فلاورجان، دانشگاه آزاد اسلامی، اصفهان، ایران

²گروه بیوتکنولوژی، واحد فلاورجان، دانشگاه آزاد اسلامی، اصفهان، ایران

³گروه میکروبیولوژی، واحد فلاورجان، دانشگاه آزاد اسلامی، اصفهان، ایران.

چکیده

تنش شوری یکی از عوامل مهم در رابطه با کاهش میزان رشد و تغییر در فرآیند‌های فیزیولوژیکی و متابولیسمی گیاهان می‌باشد. در این تحقیق جهت بررسی اثر تنش شوری بر عملکرد فیزیولوژی، بیوشیمیایی و بیان ژن در گیاه افسنطین، آزمایشی در سه سطح شوری(0، 75، 150 میلی‌مولار کلرید سدیم) در قالب طرح کاملاً تصادفی با سه تکرار در شرایط گلخانه ای انجام گرفت. بررسی نتایج نشان داد که تنش شوری باعث کاهش میزان پارامتر‌های رشدی گیاه مانند طول ساقه، طول ریشه، وزن تر ساقه، وزن تر ریشه، وزن خشک ساقه، خشک ریشه شد، همچنین شوری سبب کاهش میزان یون‌های پتاسیم، کلسیم، منیزیم و آهن و افزایش میزان سدیم در گیاه گردید. افزایش تنش شوری میزان پرولین، مالون دی آلدهید، ترکیبات فنلی و فعالیت برخی آنزیم‌های آنتی اکسیدان را افزایش و میزان پروتئین در گیاه را کاهش داد. میزان بیان ژن‌های CYP71AV1 و ADS نیز به‌ترتیب در غلظت‌های 150 میلی‌مولار و 75 میلی‌مولار کلرید سدیم بیشترین میزان کاهش را نشان داد که این امر باعث کاهش میزان آرتمیزینین در گیاه افسنطین گشت. با توجه به نتایج این پژوهش می توان عنوان نمود که گیاه افسنطین بــرای مقــابله با تنـش شوری حاصل از کلرید سدیم از سیستم افزایش فعالیت آنزیم آنتی اکسیدانی و مواد تنطیم کننده پتانسیل اسمزی و ترکیبات فنلی استفاده نموده است وکاهش بیان ژن ADS می‌تواند عامل موثر در کاهش مقدارآرتمیزینین گیاه افسنطین باشد.

کلیدواژه‌ها

آرتمیزینین؛ آنزیمهای آنتی اکسیدان؛ افسنطین؛ بیان ژن؛ تنش شوری

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